| Title: |
Assistant Professor
Visiting Researcher, Lerner Research Institute, Cleveland Clinic-OH |
| Dept: | Biological, Geological and Environmental Sciences |
| Office: | SR 260 |
| Phone: | 216-687-2409 |
| Email: | k.gulshan@csuohio.edu |
| Address: | 2121 Euclid Ave. SR 260, Cleveland, OH 44115 |
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| Education: | ||||||||
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Ph.D., Glutathione mediated detoxification pathways, Jawaharlal Nehru University, New Delhi, 2004
Postdoc, Oxidative stress response, University of Iowa, Iowa city, USA, 2008 Asst Res Scientist, ABC transporters & Lipid trafficking, University of Iowa, Iowa city, USA, 2011 | ||||||||
| Brief Bio: | ||
| Our research is focused on the role of inflammation in cardiovascular disease (CVD)and lung cancer. In the past several years, I described the role of free cholesterol in inducing autophagy and sphingomyelin biosynthesis in macrophages (PNAS 2015, Autophagy 2015). I also discovered a novel role for a minor phospholipid PIP2 in HDL biogenesis, determining that PIP2 is the lipid recognized by apoA1 in ABCA1-expressing cells, and identifying ABCA1 as a first known PIP2 floppase(Circulation Research 2016). This novel PIP2 floppase activity of ABCA1 was later confirmed by multiple studies from other labs. We identified novel anti-atherosclerotic activities of the anti-protozoan drug Miltefosine (Scientific Reports, June 2019). More recently, we demonstrated role of inflammatory protein Gasdermin D (GsdmD) in atherosclerosis by using LDLr Antisense Oligonucleotide (ASO) KO mouse model of hyperlipidemia. We showed for the first time that whole body KO of GsdmD protects against inflammation-induced defects in reverse cholesterol transport (RCT) and atherosclerosis. We showed for the first time the presence of cleaved N-terminal fragment of GsdmD in atherosclerotic plaques (Font.Cell.dev.Biol, 2021).
My lab at the Cleveland State University is equipped with Leica Microtome for tissue sectioning, QuantStudio 3 Real-Time PCR machine (ThermoFisher), SystemIBRIGHT CL750, Imaging System (Life technologies), CountessTM 3 FL Automated Cell Counter (Life technologies), bright & fluorescent microscopes for images and videos, various centrifuges, and all other standard equipments. We employ cutting edge technologies such as Crispr-Cas9, RNA Seq, 10X Single cell RNAseq, 16s rDNA seq, multiplex cytokine arrays and various mouse models of atherosclerosis and lung cancer to decipher the role of cholesterol and minor phospholipids in inflammatsome-induced CVD and cancer. The lab personnel includes one Postdoc, 2 PhD candidate graduate students, and a technician. In addition to NIH RO1 funding to study role of PIP2 in atherosclerosis and inflammation, I have obtained a Case Comprehensive Cancer Center pilot funding from Case Western University to study the role of inflammation in lung cancer progression and metastasis. I also contribute to atherosclerosis and inflammation field by performing peer review for scientific journals such as JAHA, Atherosclerosis, Autophagy, iScience (cell press), Scientific reports, Cytokine, Clinical and Experimental Allergy,Inflammatory Bowel Diseases, Cardiovascular & Hematological Disorders-Drug Targets, Drug Design, Development and Therapy, Journal of Hepatic Medicine: Evidence and Research, and served as an editor for Biomarkers of Cardiovascular Diseases; Disease Markers. In addition to research, I have experience of multiple years in teaching Microbiology with Lab, Pathophysiology, and biochemistry courses.. I also serve as an Adhoc reviewer for NIH-NHLBI study sections (INMP, NMHD, 2020-current). | ||
| Honors and Awards: | ||
| * Elsa Albretch award for best paper, 2019, Cardiovascular & Metabolic Sciences, Cleveland Clinic.
* Elsa Albretch award for best paper, 2017, Cellular & Molecular Medicine, Cleveland Clinic. * Best poster award, Gordon Research Conference of lipoprotein metabolism 2016. * Best poster award, Cleveland Clinic Institute research day 2016 (1st prize). * Best poster award, Lerner Research Institute research day 2016 (1st prize). * Best poster award, Lerner Research Institute research day 2015 (2nd prize). * Best poster award, Cleveland Clinic research day 2012 (1st prize). | ||
| Research Interests: | ||
| 1) Role of Sphingolipids in Cardiovascular disease.
2) Autophagy in heart disease and cancer. 3) Role of inflammation in heart disease. 4) Role of inflammation in lung cancer. | ||
| Teaching Areas: | ||
| Microbiology, Pathophysiology, Biochemistry. | ||
| Professional Affiliations: | ||
| 2016-2020- Member, American Heart Association (AHA)
2021-current -Member, American Society of Biochemistry and Molecular Biology (ASBMB) | ||
| Professional Experience: | ||
| 2005-2011: Assistant Research Scientist, University of Iowa, Iowa-city, IA.
2011- 2015:Research Associate, Lerner Research Institute, Cleveland Clinic, OH 2015-2016:Project Scientist, Lerner Research Institute, Cleveland Clinic, OH 2016-2020: Adjunct Assistant Professor of Molecular Medicine, Cleveland Clinic, OH | ||
| University Service: | ||
| Member of CSU IACUC committee (2021-current).
Teaching Biochem courses (2020-current) | ||
| Professional Service: | ||
| Grant review for NIH, and manuscripts review for various peer-reviewed journals. | ||
| Community Service: | ||
| Judging scientific posters/events:
Annual Concept Science & Engineering Fair (CONSEF), Cleveland (2014-2017). NEOSEF, Cleveland State University (2016). Solon Middle School Science Fair, Solon, OH (2017) | ||
| Research Grants: | ||
| Active:
1) NIH-NHLBI-RO1: (K Gulshan, PI) (04/01/2020-03/31/2024) Title: Phosphatidylinositol metabolism and trafficking in atherosclerosis and inflammation. 2) Case Comprehensive Cancer Center: (K Gulshan, PI) (06/2020-06/2021) Title: Role of PIP2 and Gasdermin D in Lung Cancer Progression and Metastasis. Completed: 1) American Heart Association Scientist Development Grant SDG25710128 (K Gulshan, PI) (7/1/2015-6/30/2019) Title: Mechanisms of cellular lipid homeostasis and nascent HDL formation. 2) NCAI-CC-2016-I-Corps-SD (K Gulshan, PI) (6/1/2016-7/31/2016). | ||